Multiple forms of membrane-bound beta-glucosidase in human leucocytes.

insulin has been suggested by Cuatrecasas & Illiano (197 1) on the basis of neuraminidase treatment of adipose-tissue cells. Enzymic digestion was reported to have no effect on the total quantity of insulin receptor in the membranes or on the affinity of the receptor for insulin, but abolished the effect of insulin on glucose transport and on lipolysis. As shown in Fig. 1, neuraminidase action inhibits the observed insulin effects on chick erythrocyte mobility, and this would be consistent with the suggestion of a dissociation of the electrophoretic effects and receptor-binding properties of the molecule.